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Here is the published sequence for the portion of the PTC gene that you will amplify today.
5’- CCTTCGTTTTCTTGGTGAATTTTTGGGATGTAGTGAAGA
GGCGGCCACTGAGCAACAGTGATTGTGTGCTGCTGTGTC
TCAGCATCAGCCGGCTTTTCCTGCATGGACTGCTGTTCC
TGAGTGCTATCCAGCTTACCCACTTCCAGAAGTTGAGTG
AACCACTGAACCACAGCTACCAAGCCATCATCATGCTAT
GGATGATTGCAAACCAAGCCAACCT -3’
Here is the sequence for the forward/right primer.5’
- CCTTCGTTTTCTTGGTGAATTTTT -3
Underneath the published sequence, write the complementary strand of DNA.Indicate the 5’ and 3’ end of this strand.



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